Keith Humphries, MD, PhD
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Professor Department of Medicine, University of British Columbia Associate Member, Department of Medical Genetics, UBC Associate Member, Pathology & Laboratory Medicine, UBC Deputy Director, Terry Fox Laboratory Senior Scientist, Terry Fox Laboratory Program Project Group Leader, Terry Fox Foundation of Canada Director, Transgenic and Gene Targeting Facility, BCCA Member, Stem Cell Network Tel: 604-675-8140 Email:  |
Selected Publications:
Heuser M, Yap DB, Leung M, de Algara TR, Tafech A, McKinney S, Dixon J, Thresher R, Colledge B, Carlton M, Humphries RK, & Aparicio SA. Loss of MLL5 results in pleiotropic hematopoietic defects, reduced neutrophil immune function, and extreme sensitivity to DNA demethylation. Blood 113 (7): 1432-43, 2009.
Heuser M, Sly LM, Argiropoulos B, Kuchenbauer F, Lai C, Weng A, Leung M, Lin G, Brookes C, Fung S, Valk PJ, Delwel R, Lowenberg B, Krystal G, & Humphries RK. Modelling the functional heterogeneity of leukemia stem cells: role of STAT5 in leukemia stem cell self-renewal. Blood, 2009.
Heuser M, Kuchenbauer F, Argiropoulos B, Sekulovic S, Leung M, Stasiak M, Ganser A, & Humphries RK. Priming reloaded? Blood 114 (4): 925-6; author reply 926-7, 2009.
Sekulovic S, Imren S & Humphries K. High level in vitro expansion of murine hematopoietic stem cells. In:Current Protocols in Stem Cell Biology. John Wiley & Sons Inc.,NJ., p Unit 2A.7., 2008.
Faubert A, Chagraoui J, Mayotte N, Fréchette M, Iscove NN, Humphries RK & Sauvageau G. Complementary and independent function for Hoxb4 and Bmi1 in HSC activity. Cold Spring Harb Symp Quant Biol 2008.
Heuser M*, Yap D*, Leung M, de Algara TR, Tafech A, McKinney S, Dixon J, Thresher R, Colledge B, Carlton M, Humphries RK** & Aparicio S**. Loss of MLL5 results in pleiotropic hematopoietic defects, reduced neutrophil immune function and extreme sensitivity to DNA demethylation. Blood 2008 (*authors contributed equally to this study; **co-senior authors).
Kuchenbauer F*, Morin RD*, Argiropoulos B, Petriv OI, Griffith M, Heuser M, Yung E, Piper J, Delaney A, Prabhu A-L, Zhao Y, McDonald H, Zeng T, Hirst M, Hansen CL, Marra MA & Humphries RK. In-depth characterization of the microRNA transcriptome in a leukemia progression model .Genome Res 18: 1787-97, 2008. (*authors contributed equally to this study).
Argiropoulos B, Palmqvist L, Yung E, Kuchenbauer F, Heuser M, Sly LM, Wan A, Krystal G, & Humphries RK. Linkage of Meis1 leukemogenic activity to multiple downstream effectors including Trib2 and Ccl3. Exp Hematol 36 (7): 845-59, 2008.
Hirose K, Abramovich C, Argiropoulos B, & Humphries RK. Leukemogenic properties of NUP98-PMX1 are linked to NUP98 and homeodomain sequence functions but not to binding properties of PMX1 to serum response factor. Oncogene, 2008.
Rouhi A, Mager DL, Humphries RK, & Kuchenbauer F. MiRNAs, epigenetics, and cancer. Mamm Genome, 2008.
Zhang XB, Beard BC, Trobridge GD, Wood BL, Sale GE, Sud R, Humphries RK, & Kiem HP. High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector. J Clin Invest 118 (4): 1502-10, 2008.
Argiropoulos B, & Humphries RK. Hox genes in hematopoiesis and leukemogenesis. Oncogene 26 (47): 6766-76, 2007.
Argiropoulos B, Yung E, & Humphries RK. Unraveling the crucial roles of Meis1 in leukemogenesis and normal hematopoiesis. Genes Dev 21 (22): 2845-9, 2007.
Cellot S, Krosl J, Chagraoui J, Meloche S, Humphries RK, & Sauvageau G. Sustained in vitro trigger of self-renewal divisions in Hoxb4hiPbx1(10) hematopoietic stem cells. Exp Hematol 35 (5): 802-16, 2007.
Heuser M, Argiropoulos B, Kuchenbauer F, Yung E, Piper J, Fung S, Schlenk RF, Dohner K, Hinrichsen T, Rudolph C, Schambach A, Baum C, Schlegelberger B, Dohner H, Ganser A, & Humphries RK. MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML. Blood 110 (5): 1639-47, 2007.
Lu M, Glover CH, Tien AH, Humphries RK, Piret JM, & Helgason CD. Involvement of tyrosine kinase signaling in maintaining murine embryonic stem cell functionality. Exp Hematol 35 (8): 1293-302, 2007.
Ohta H, Sekulovic S, Bakovic S, Eaves CJ, Pineault N, Gasparetto M, Smith C, Sauvageau G, & Humphries RK. Near-maximal expansions of hematopoietic stem cells in culture using NUP98-HOX fusions. Exp Hematol 35 (5): 817-30, 2007.
Palmqvist L, Pineault N, Wasslavik C, & Humphries RK. Candidate genes for expansion and transformation of hematopoietic stem cells by NUP98-HOX fusion genes. PLoS ONE 2 (1): e768, 2007.
Zhang XB, Schwartz JL, Humphries RK, & Kiem HP. Effects of HOXB4 overexpression on ex vivo expansion and immortalization of hematopoietic cells from different species. Stem Cells 25 (8): 2074-81, 2007.
Deshpande AJ, Cusan M, Rawat VP, Reuter H, Krause A, Pott C, Quintanilla-Martinez L, Kakadia P, Kuchenbauer F, Ahmed F, Delabesse E, Hahn M, Lichter P, Kneba M, Hiddemann W, Macintyre E, Mecucci C, Ludwig WD, Humphries RK, Bohlander SK, Feuring-Buske M, & Buske C. Acute myeloid leukemia is propagated by a leukemic stem cell with lymphoid characteristics in a mouse model of CALM/AF10-positive leukemia. Cancer Cell 10 (5): 363-74, 2006.
Fisher CL, Randazzo F, Humphries RK, & Brock HW. Characterization of Asxl1, a murine homolog of Additional sex combs, and analysis of the Asx-like gene family. Gene 369: 109-18, 2006.
Gurevich RM, Rosten PM, Schwieger M, Stocking C, & Humphries RK. Retroviral integration site analysis identifies ICSBP as a collaborating tumor suppressor gene in NUP98-TOP1-induced leukemia. Exp Hematol 34 (9): 1192-201, 2006.
Palmqvist L, Argiropoulos B, Pineault N, Abramovich C, Sly LM, Krystal G, Wan A, & Humphries RK. The Flt3 receptor tyrosine kinase collaborates with NUP98-HOX fusions in acute myeloid leukemia. Blood 108 (3): 1030-6, 2006.
Zhang XB, Beard BC, Beebe K, Storer B, Humphries RK, & Kiem HP. Differential effects of HOXB4 on nonhuman primate short- and long-term repopulating cells. PLoS Med 3 (5): e173, 2006.
Abramovich C, & Humphries RK. Hox regulation of normal and leukemic hematopoietic stem cells. Curr Opin Hematol 12 (3): 210-6, 2005.
Abramovich C, Pineault N, Ohta H, & Humphries RK. Hox genes: from leukemia to hematopoietic stem cell expansion. Ann N Y Acad Sci 1044: 109-16, 2005.
Ferrell CM, Dorsam ST, Ohta H, Humphries RK, Derynck MK, Haqq C, Largman C, & Lawrence HJ. Activation of stem-cell specific genes by HOXA9 and HOXA10 homeodomain proteins in CD34+ human cord blood cells. Stem Cells 23 (5): 644-55, 2005.
Lawrence HJ, Christensen J, Fong S, Hu YL, Weissman I, Sauvageau G, Humphries RK, & Largman C. Loss of expression of the Hoxa-9 homeobox gene impairs the proliferation and repopulating ability of hematopoietic stem cells. Blood 106 (12): 3988-94, 2005.
Milsom MD, Duxbury R, Gagen D, Humphries RK, Schmidt M, von-Kalle C, & Fairbairn LJ. Overexpression of HOXB4 confers a myelo-erythroid differentiation delay in vitro. Leukemia 19 (1): 148-53, 2005.
Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, & Helgason CD. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells 23 (5): 663-80, 2005.
Pineault N, Abramovich C, & Humphries RK. Transplantable cell lines generated with NUP98-Hox fusion genes undergo leukemic progression by Meis1 independent of its binding to DNA. Leukemia 19 (4): 636-43, 2005.
Schessl C, Rawat VP, Cusan M, Deshpande A, Kohl TM, Rosten PM, Spiekermann K, Humphries RK, Schnittger S, Kern W, Hiddemann W, Quintanilla-Martinez L, Bohlander SK, Feuring-Buske M, & Buske C. The AML1-ETO fusion gene and the FLT3 length mutation collaborate in inducing acute leukemia in mice. J Clin Invest 115 (8): 2159-68, 2005.
Gurevich RM, Aplan PD, & Humphries RK. NUP98-topoisomerase I acute myeloid leukemia-associated fusion gene has potent leukemogenic activities independent of an engineered catalytic site mutation. Blood 104 (4): 1127-36, 2004.
Imren S, Fabry ME, Westerman KA, Pawliuk R, Tang P, Rosten PM, Nagel RL, Leboulch P, Eaves CJ, & Humphries RK. High-level beta-globin expression and preferred intragenic integration after lentiviral transduction of human cord blood stem cells. J Clin Invest 114 (7): 953-62, 2004.
Milsom MD, Woolford LB, Margison GP, Humphries RK, & Fairbairn LJ. Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant O6-methylguanine-DNA-methyltransferase and HOXB4. Mol Ther 10 (5): 862-73, 2004.
Nicolini FE, Cashman JD, Hogge DE, Humphries RK, & Eaves CJ. NOD/SCID mice engineered to express human IL-3, GM-CSF and Steel factor constitutively mobilize engrafted human progenitors and compromise human stem cell regeneration. Leukemia 18 (2): 341-7, 2004.
Oh IH, Fabry ME, Humphries RK, Pawliuk R, Leboulch P, Hoffman R, Nagel RL, & Eaves C. Expression of an anti-sickling beta-globin in human erythroblasts derived from retrovirally transduced primitive normal and sickle cell disease hematopoietic cells. Exp Hematol 32 (5): 461-9, 2004.
Pineault N, Abramovich C, Ohta H, & Humphries RK. Differential and common leukemogenic potentials of multiple NUP98-Hox fusion proteins alone or with Meis1. Mol Cell Biol 24 (5): 1907-17, 2004.
Rawat VP, Cusan M, Deshpande A, Hiddemann W, Quintanilla-Martinez L, Humphries RK, Bohlander SK, Feuring-Buske M, & Buske C. Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia. Proc Natl Acad Sci U S A 101 (3): 817-22, 2004.
Sauvageau G, Iscove NN, & Humphries RK. In vitro and in vivo expansion of hematopoietic stem cells. Oncogene 23 (43): 7223-32, 2004.
Baran CP, Tridandapani S, Helgason CD, Humphries RK, Krystal G, & Marsh CB. The inositol 5’-phosphatase SHIP-1 and the Src kinase Lyn negatively regulate macrophage colony-stimulating factor-induced Akt activity. J Biol Chem 278 (40): 38628-36, 2003.
Bjornsson JM, Larsson N, Brun AC, Magnusson M, Andersson E, Lundstrom P, Larsson J, Repetowska E, Ehinger M, Humphries RK, & Karlsson S. Reduced proliferative capacity of hematopoietic stem cells deficient in Hoxb3 and Hoxb4. Mol Cell Biol 23 (11): 3872-83, 2003.
Brun AC, Fan X, Bjornsson JM, Humphries RK, & Karlsson S. Enforced adenoviral vector-mediated expression of HOXB4 in human umbilical cord blood CD34+ cells promotes myeloid differentiation but not proliferation. Mol Ther 8 (4): 618-28, 2003.
Crow AR, Song S, Freedman J, Helgason CD, Humphries RK, Siminovitch KA, & Lazarus AH. IVIg-mediated amelioration of murine ITP via FcgammaRIIB is independent of SHIP1, SHP-1, and Btk activity. Blood 102 (2): 558-60, 2003.
Feuring-Buske M, Gerhard B, Cashman J, Humphries RK, Eaves CJ, & Hogge DE. Improved engraftment of human acute myeloid leukemia progenitor cells in beta 2-microglobulin-deficient NOD/SCID mice and in NOD/SCID mice transgenic for human growth factors. Leukemia 17 (4): 760-3, 2003.
Helgason CD, Antonchuk J, Bodner C, & Humphries RK. Homeostasis and regeneration of the hematopoietic stem cell pool are altered in SHIP-deficient mice. Blood 102 (10): 3541-7, 2003.
Krosl J, Austin P, Beslu N, Kroon E, Humphries RK, & Sauvageau G. In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein. Nat Med 9 (11): 1428-32, 2003.
Krosl J, Beslu N, Mayotte N, Humphries RK, & Sauvageau G. The competitive nature of HOXB4-transduced HSC is limited by PBX1: the generation of ultra-competitive stem cells retaining full differentiation potential. Immunity 18 (4): 561-71, 2003.
Larrivee B, Lane DR, Pollet I, Olive PL, Humphries RK, & Karsan A. Vascular endothelial growth factor receptor-2 induces survival of hematopoietic progenitor cells. J Biol Chem 278 (24): 22006-13, 2003.
Pineault N, Buske C, Feuring-Buske M, Abramovich C, Rosten P, Hogge DE, Aplan PD, & Humphries RK. Induction of acute myeloid leukemia in mice by the human leukemia-specific fusion gene NUP98-HOXD13 in concert with Meis1. Blood 101 (11): 4529-38, 2003.
Abramovich C, Chavez EA, Lansdorp PM, & Humphries RK. Functional characterization of multiple domains involved in the subcellular localization of the hematopoietic Pbx interacting protein (HPIP). Oncogene 21 (44): 6766-71, 2002.
Antonchuk J, Sauvageau G, & Humphries RK. HOXB4-induced expansion of adult hematopoietic stem cells ex vivo. Cell 109 (1): 39-45, 2002.
Buske C, Feuring-Buske M, Abramovich C, Spiekermann K, Eaves CJ, Coulombel L, Sauvageau G, Hogge DE, & Humphries RK. Deregulated expression of HOXB4 enhances the primitive growth activity of human hematopoietic cells. Blood 100 (3): 862-8, 2002.
Imren S, Payen E, Westerman KA, Pawliuk R, Fabry ME, Eaves CJ, Cavilla B, Wadsworth LD, Beuzard Y, Bouhassira EE, Russell R, London IM, Nagel RL, Leboulch P, & Humphries RK. Permanent and panerythroid correction of murine beta thalassemia by multiple lentiviral integration in hematopoietic stem cells. Proc Natl Acad Sci U S A 99 (22): 14380-5, 2002.
Miller CL, Imren S, Antonchuk J, Kalberer C, Fabry ME, Nagel RL, Humphries RK, & Eaves CJ. Feasibility of using autologous transplantation to evaluate hematopoietic stem cell-based gene therapy strategies in transgenic mouse models of human disease. Mol Ther 6 (3): 422-8, 2002.
Nicolini FE, Imren S, Oh IH, Humphries RK, Leboulch P, Fabry ME, Nagel RL, & Eaves CJ. Expression of a human beta-globin transgene in erythroid cells derived from retrovirally transduced transplantable human fetal liver and cord blood cells. Blood 100 (4): 1257-64, 2002.
Ohta H, Sawada A, Kim JY, Tokimasa S, Nishiguchi S, Humphries RK, Hara J, & Takihara Y. Polycomb group gene rae28 is required for sustaining activity of hematopoietic stem cells. J Exp Med 195 (6): 759-70, 2002.
Pineault N, Helgason CD, Lawrence HJ, & Humphries RK. Differential expression of Hox, Meis1, and Pbx1 genes in primitive cells throughout murine hematopoietic ontogeny. Exp Hematol 30 (1): 49-57, 2002.
Takeshita S, Namba N, Zhao JJ, Jiang Y, Genant HK, Silva MJ, Brodt MD, Helgason CD, Kalesnikoff J, Rauh MJ, Humphries RK, Krystal G, Teitelbaum SL, & Ross FP. SHIP-deficient mice are severely osteoporotic due to increased numbers of hyper-resorptive osteoclasts. Nat Med 8 (9): 943-9, 2002.
Thorsteinsdottir U, Mamo A, Kroon E, Jerome L, Bijl J, Lawrence HJ, Humphries K, & Sauvageau G. Overexpression of the myeloid leukemia-associated Hoxa9 gene in bone marrow cells induces stem cell expansion. Blood 99 (1): 121-9, 2002.
Antonchuk J, Sauvageau G, & Humphries RK. HOXB4 overexpression mediates very rapid stem cell regeneration and competitive hematopoietic repopulation. Exp Hematol 29 (9): 1125-34, 2001.
Bjornsson JM, Andersson E, Lundstrom P, Larsson N, Xu X, Repetowska E, Humphries RK, & Karlsson S. Proliferation of primitive myeloid progenitors can be reversibly induced by HOXA10. Blood 98 (12): 3301-8, 2001.
Buske C, Feuring-Buske M, Antonchuk J, Rosten P, Hogge DE, Eaves CJ, & Humphries RK. Overexpression of HOXA10 perturbs human lymphomyelopoiesis in vitro and in vivo. Blood 97 (8): 2286-92, 2001.
Latour S, Gish G, Helgason CD, Humphries RK, Pawson T, & Veillette A. Regulation of SLAM-mediated signal transduction by SAP, the X-linked lymphoproliferative gene product. Nat Immunol 2 (8): 681-90, 2001.
Pawliuk R, Westerman KA, Fabry ME, Payen E, Tighe R, Bouhassira EE, Acharya SA, Ellis J, London IM, Eaves CJ, Humphries RK, Beuzard Y, Nagel RL, & Leboulch P. Correction of sickle cell disease in transgenic mouse models by gene therapy. Science 294 (5550): 2368-71, 2001.
Rufer N, Migliaccio M, Antonchuk J, Humphries RK, Roosnek E, & Lansdorp PM. Transfer of the human telomerase reverse transcriptase (TERT) gene into T lymphocytes results in extension of replicative potential. Blood 98 (3): 597-603, 2001.
Abramovich C, Shen WF, Pineault N, Imren S, Montpetit B, Largman C, & Humphries RK. Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA. J Biol Chem 275 (34): 26172-7, 2000.
Brauweiler A, Tamir I, Dal Porto J, Benschop RJ, Helgason CD, Humphries RK, Freed JH, & Cambier JC. Differential regulation of B cell development, activation, and death by the src homology 2 domain-containing 5’ inositol phosphatase (SHIP). J Exp Med 191 (9): 1545-54, 2000.
Buske C, & Humphries RK. Homeobox genes in leukemogenesis. Int J Hematol 71 (4): 301-8, 2000.
Helgason CD, Kalberer CP, Damen JE, Chappel SM, Pineault N, Krystal G, & Humphries RK. A dual role for Src homology 2 domain-containing inositol-5-phosphatase (SHIP) in immunity: aberrant development and enhanced function of b lymphocytes in ship -/- mice. J Exp Med 191 (5): 781-94, 2000.
Hennemann B, Chuo JY, Schley PD, Lambie K, Humphries RK, & Eaves CJ. High-efficiency retroviral transduction of mammalian cells on positively charged surfaces. Hum Gene Ther 11 (1): 43-51, 2000.
Hennemann B, Oh IH, Chuo JY, Kalberer CP, Schley PD, Rose-John S, Humphries RK, & Eaves CJ. Efficient retrovirus-mediated gene transfer to transplantable human bone marrow cells in the absence of fibronectin. Blood 96 (7): 2432-9, 2000.
Kalberer CP, Pawliuk R, Imren S, Bachelot T, Takekoshi KJ, Fabry M, Eaves CJ, London IM, Humphries RK, & Leboulch P. Preselection of retrovirally transduced bone marrow avoids subsequent stem cell gene silencing and age-dependent extinction of expression of human beta-globin in engrafted mice. Proc Natl Acad Sci U S A 97 (10): 5411-5, 2000.
Lu L, Chappel MS, Humphries RK, & Osmond DG. Regulation of cell survival during B lymphopoiesis: increased pre-B cell apoptosis in CD24-transgenic mouse bone marrow. Eur J Immunol 30 (9): 2686-91, 2000.
Metzler M, Helgason CD, Dragatsis I, Zhang T, Gan L, Pineault N, Zeitlin SO, Humphries RK, & Hayden MR. Huntingtin is required for normal hematopoiesis. Hum Mol Genet 9 (3): 387-94, 2000.
Satterthwaite AB, Willis F, Kanchanastit P, Fruman D, Cantley LC, Helgason CD, Humphries RK, Lowell CA, Simon M, Leitges M, Tarakhovsky A, Tedder TF, Lesche R, Wu H, & Witte ON. A sensitized genetic system for the analysis of murine B lymphocyte signal transduction pathways dependent on Bruton’s tyrosine kinase. Proc Natl Acad Sci U S A 97 (12): 6687-92, 2000.
Ailles LE, Humphries RK, Thomas TE, & Hogge DE. Retroviral marking of acute myelogenous leukemia progenitors that initiate long-term culture and growth in immunodeficient mice. Exp Hematol 27 (11): 1609-20, 1999.
Doheny JG, Jervis EJ, Guarna MM, Humphries RK, Warren RA, & Kilburn DG. Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor-cellulose-binding domain fusion protein. Biochem J 339 ( Pt 2): 429-34, 1999.
Hennemann B, Conneally E, Pawliuk R, Leboulch P, Rose-John S, Reid D, Chuo JY, Humphries RK, & Eaves CJ. Optimization of retroviral-mediated gene transfer to human NOD/SCID mouse repopulating cord blood cells through a systematic analysis of protocol variables. Exp Hematol 27 (5): 817-25, 1999.
Huber M, Helgason CD, Damen JE, Scheid M, Duronio V, Liu L, Ware MD, Humphries RK, & Krystal G. The role of SHIP in growth factor induced signalling. Prog Biophys Mol Biol 71 (3-4): 423-34, 1999.
Huber M, Helgason CD, Damen JE, Scheid MP, Duronio V, Lam V, Humphries RK, & Krystal G. The role of the SRC homology 2-containing inositol 5’-phosphatase in Fc epsilon R1-induced signaling. Curr Top Microbiol Immunol 244: 29-41, 1999.
Kawagoe H, Humphries RK, Blair A, Sutherland HJ, & Hogge DE. Expression of HOX genes, HOX cofactors, and MLL in phenotypically and functionally defined subpopulations of leukemic and normal human hematopoietic cells. Leukemia 13 (5): 687-98, 1999.
Kim CH, Hangoc G, Cooper S, Helgason CD, Yew S, Humphries RK, Krystal G, & Broxmeyer HE. Altered responsiveness to chemokines due to targeted disruption of SHIP. J Clin Invest 104 (12): 1751-9, 1999.
Krystal G, Damen JE, Helgason CD, Huber M, Hughes MR, Kalesnikoff J, Lam V, Rosten P, Ware MD, Yew S, & Humphries RK. SHIPs ahoy. Int J Biochem Cell Biol 31 (10): 1007-10, 1999.
Metzler M, Chen N, Helgason CD, Graham RK, Nichol K, McCutcheon K, Nasir J, Humphries RK, Raymond LA, & Hayden MR. Life without huntingtin: normal differentiation into functional neurons. J Neurochem 72 (3): 1009-18, 1999.
Thorsteinsdottir U, Sauvageau G, & Humphries RK. Enhanced in vivo regenerative potential of HOXB4-transduced hematopoietic stem cells with regulation of their pool size. Blood 94 (8): 2605-12, 1999.
Conneally E, Eaves CJ, & Humphries RK. Efficient retroviral-mediated gene transfer to human cord blood stem cells with in vivo repopulating potential. Blood 91 (9): 3487-93, 1998.
Helgason CD, Damen JE, Rosten P, Grewal R, Sorensen P, Chappel SM, Borowski A, Jirik F, Krystal G, & Humphries RK. Targeted disruption of SHIP leads to hemopoietic perturbations, lung pathology, and a shortened life span. Genes Dev 12 (11): 1610-20, 1998.
Huber M, Helgason CD, Damen JE, Liu L, Humphries RK, & Krystal G. The src homology 2-containing inositol phosphatase (SHIP) is the gatekeeper of mast cell degranulation. Proc Natl Acad Sci U S A 95 (19): 11330-5, 1998.
Huber M, Helgason CD, Scheid MP, Duronio V, Humphries RK, & Krystal G. Targeted disruption of SHIP leads to Steel factor-induced degranulation of mast cells. Embo J 17 (24): 7311-9, 1998.
Pawliuk R, Bachelot T, Raftopoulos H, Kalberer C, Humphries RK, Bank A, & Leboulch P. Retroviral vectors aimed at the gene therapy of human beta-globin gene disorders. Ann N Y Acad Sci 850: 151-62, 1998.
Bardy P, Conneally E, Emerman JT, Lansdorp PM, Goss G, Humphries RK, & Eaves CJ. Isolation and analysis of different subpopulations of normal human breast epithelial cells after their infection with a retroviral vector encoding a cell surface marker. Breast Cancer Res Treat 44 (2): 153-65, 1997.
Damen JE, Liu L, Wakao H, Miyajima A, Rosten P, Jefferson AB, Majerus PW, Krosl J, Humphries RK, & Krystal G. The role of erythropoietin receptor tyrosine phosphorylation in erythropoietin-induced proliferation. Leukemia 11 Suppl 3: 423-5, 1997.
Lawrence HJ, Helgason CD, Sauvageau G, Fong S, Izon DJ, Humphries RK, & Largman C. Mice bearing a targeted interruption of the homeobox gene HOXA9 have defects in myeloid, erythroid, and lymphoid hematopoiesis. Blood 89 (6): 1922-30, 1997.
Pawliuk R, Eaves CJ, & Humphries RK. Sustained high-level reconstitution of the hematopoietic system by preselected hematopoietic cells expressing a transduced cell-surface antigen. Hum Gene Ther 8 (13): 1595-604, 1997.
Sauvageau G, Thorsteinsdottir U, Hough MR, Hugo P, Lawrence HJ, Largman C, & Humphries RK. Overexpression of HOXB3 in hematopoietic cells causes defective lymphoid development and progressive myeloproliferation. Immunity 6 (1): 13-22, 1997.
Thorsteinsdottir U, Sauvageau G, Hough MR, Dragowska W, Lansdorp PM, Lawrence HJ, Largman C, & Humphries RK. Overexpression of HOXA10 in murine hematopoietic cells perturbs both myeloid and lymphoid differentiation and leads to acute myeloid leukemia. Mol Cell Biol 17 (1): 495-505, 1997.
Thorsteinsdottir U, Sauvageau G, & Humphries RK. Hox homeobox genes as regulators of normal and leukemic hematopoiesis. Hematol Oncol Clin North Am 11 (6): 1221-37, 1997.
Chappel MS, Hough MR, Mittel A, Takei F, Kay R, & Humphries RK. Cross-linking the murine heat-stable antigen induces apoptosis in B cell precursors and suppresses the anti-CD40-induced proliferation of mature resting B lymphocytes. J Exp Med 184 (5): 1638-49, 1996.
Conneally E, Bardy P, Eaves CJ, Thomas T, Chappel S, Shpall EJ, & Humphries RK. Rapid and efficient selection of human hematopoietic cells expressing murine heat-stable antigen as an indicator of retroviral-mediated gene transfer. Blood 87 (2): 456-64, 1996.
Damen JE, Liu L, Rosten P, Humphries RK, Jefferson AB, Majerus PW, & Krystal G. The 145-kDa protein induced to associate with Shc by multiple cytokines is an inositol tetraphosphate and phosphatidylinositol 3,4,5-triphosphate 5-phosphatase. Proc Natl Acad Sci U S A 93 (4): 1689-93, 1996.
Helgason CD, Sauvageau G, Lawrence HJ, Largman C, & Humphries RK. Overexpression of HOXB4 enhances the hematopoietic potential of embryonic stem cells differentiated in vitro. Blood 87 (7): 2740-9, 1996.
Hough MR, Chappel MS, Sauvageau G, Takei F, Kay R, & Humphries RK. Reduction of early B lymphocyte precursors in transgenic mice overexpressing the murine heat-stable antigen. J Immunol 156 (2): 479-88, 1996.
Krosl J, Damen JE, Krystal G, & Humphries RK. Interleukin-3 (IL-3) inhibits erythropoietin-induced differentiation in Ba/F3 cells via the IL-3 receptor alpha subunit. J Biol Chem 271 (44): 27432-7, 1996.
Lawrence HJ, Sauvageau G, Humphries RK, & Largman C. The role of HOX homeobox genes in normal and leukemic hematopoiesis. Stem Cells 14 (3): 281-91, 1996.
Medin JA, Migita M, Pawliuk R, Jacobson S, Amiri M, Kluepfel-Stahl S, Brady RO, Humphries RK, & Karlsson S. A bicistronic therapeutic retroviral vector enables sorting of transduced CD34+ cells and corrects the enzyme deficiency in cells from Gaucher patients. Blood 87 (5): 1754-62, 1996.
Pawliuk R, Eaves C, & Humphries RK. Evidence of both ontogeny and transplant dose-regulated expansion of hematopoietic stem cells in vivo. Blood 88 (8): 2852-8, 1996.
Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, & Largman C. Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Nucleic Acids Res 24 (5): 898-906, 1996.
Ware MD, Rosten P, Damen JE, Liu L, Humphries RK, & Krystal G. Cloning and characterization of human SHIP, the 145-kD inositol 5-phosphatase that associates with SHC after cytokine stimulation. Blood 88 (8): 2833-40, 1996.
Damen JE, Wakao H, Miyajima A, Krosl J, Humphries RK, Cutler RL, & Krystal G. Tyrosine 343 in the erythropoietin receptor positively regulates erythropoietin-induced cell proliferation and Stat5 activation. Embo J 14 (22): 5557-68, 1995.
Krosl J, Damen JE, Krystal G, & Humphries RK. Erythropoietin and interleukin-3 induce distinct events in erythropoietin receptor-expressing BA/F3 cells. Blood 85 (1): 50-6, 1995.
Krystal G, Humphries K, & Damen J. A second erythropoietin receptor subunit. Blood 85 (9): 2641, 1995.
Lawrence HJ, Sauvageau G, Ahmadi N, Lopez AR, LeBeau MM, Link M, Humphries K, & Largman C. Stage- and lineage-specific expression of the HOXA10 homeobox gene in normal and leukemic hematopoietic cells. Exp Hematol 23 (11): 1160-6, 1995.
Lewis ME, Forsythe IJ, Marth JD, Brunzell JD, Hayden MR, & Humphries RK. Retroviral-mediated gene transfer and expression of human lipoprotein lipase in somatic cells. Hum Gene Ther 6 (7): 853-63, 1995.
Migita M, Medin JA, Pawliuk R, Jacobson S, Nagle JW, Anderson S, Amiri M, Humphries RK, & Karlsson S. Selection of transduced CD34+ progenitors and enzymatic correction of cells from Gaucher patients, with bicistronic vectors. Proc Natl Acad Sci U S A 92 (26): 12075-9, 1995.
Sauvageau G, Thorsteinsdottir U, Eaves CJ, Lawrence HJ, Largman C, Lansdorp PM, & Humphries RK. Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo. Genes Dev 9 (14): 1753-65, 1995.
Barnett MJ, Eaves CJ, Phillips GL, Gascoyne RD, Hogge DE, Horsman DE, Humphries RK, Klingemann HG, Lansdorp PM, Nantel SH, & et al. Autografting with cultured marrow in chronic myeloid leukemia: results of a pilot study. Blood 84 (3): 724-32, 1994.
Hogge DE, Sutherland HJ, Cashman JD, Lansdorp PM, Humphries RK, & Eaves CJ. Cytokines acting early in human haematopoiesis. Baillieres Clin Haematol 7 (1): 49-63, 1994.
Hough MR, Rosten PM, Sexton TL, Kay R, & Humphries RK. Mapping of CD24 and homologous sequences to multiple chromosomal loci. Genomics 22 (1): 154-61, 1994.
Hough MR, Takei F, Humphries RK, & Kay R. Defective development of thymocytes overexpressing the costimulatory molecule, heat-stable antigen. J Exp Med 179 (1): 177-84, 1994.
Leboulch P, Huang GM, Humphries RK, Oh YH, Eaves CJ, Tuan DY, & London IM. Mutagenesis of retroviral vectors transducing human beta-globin gene and beta-globin locus control region derivatives results in stable transmission of an active transcriptional structure. Embo J 13 (13): 3065-76, 1994.
Pawliuk R, Kay R, Lansdorp P, & Humphries RK. Selection of retrovirally transduced hematopoietic cells using CD24 as a marker of gene transfer. Blood 84 (9): 2868-77, 1994.
Rebel VI, Dragowska W, Eaves CJ, Humphries RK, & Lansdorp PM. Amplification of Sca-1+ Lin- WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential. Blood 83 (1): 128-36, 1994.
Sauvageau G, Lansdorp PM, Eaves CJ, Hogge DE, Dragowska WH, Reid DS, Largman C, Lawrence HJ, & Humphries RK. Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells. Proc Natl Acad Sci U S A 91 (25): 12223-7, 1994.
Fraser CC, Thacker JD, Hogge DE, Fatur-Saunders D, Takei F, & Humphries RK. Alterations in lymphopoiesis after hematopoietic reconstitution with IL-7 virus-infected bone marrow. J Immunol 151 (5): 2409-18, 1993.
Damen J, Mui AL, Hughes P, Humphries K, & Krystal G. Erythropoietin-induced tyrosine phosphorylations in a high erythropoietin receptor-expressing lymphoid cell line. Blood 80 (8): 1923-32, 1992.
Eaves CJ, Sutherland HJ, Udomsakdi C, Lansdorp PM, Szilvassy SJ, Fraser CC, Humphries RK, Barnett MJ, Phillips GL, & Eaves AC. The human hematopoietic stem cell in vitro and in vivo. Blood Cells 18 (2): 301-7, 1992.
Fraser CC, Szilvassy SJ, Eaves CJ, & Humphries RK. Proliferation of totipotent hematopoietic stem cells in vitro with retention of long-term competitive in vivo reconstituting ability. Proc Natl Acad Sci U S A 89 (5): 1968-72, 1992.
Hughes PF, Thacker JD, Hogge D, Sutherland HJ, Thomas TE, Lansdorp PM, Eaves CJ, & Humphries RK. Retroviral gene transfer to primitive normal and leukemic hematopoietic cells using clinically applicable procedures. J Clin Invest 89 (6): 1817-24, 1992.
Mui AL, Kay RJ, Humphries RK, & Krystal G. Ligand-induced phosphorylation of the murine interleukin 3 receptor signals its cleavage. Proc Natl Acad Sci U S A 89 (22): 10812-6, 1992.
Mui AL, Kay RJ, Humphries RK, & Krystal G. Purification of the murine interleukin 3 receptor. J Biol Chem 267 (23): 16523-30, 1992.
Munt B, Erb S, Humphries K, Shepherd J, Scudamore C, Anderson F, Lam V, Shackleton C, & Krystal G. Monitoring hemangioblastoma tumor burden using plasma erythropoietin levels. Int J Hematol 56 (2): 185-7, 1992.
Turhan AG, Cashman JD, Eaves CJ, Humphries RK, & Eaves AC. Variable expression of features of normal and neoplastic stem cells in patients with thrombocytosis. Br J Haematol 82 (1): 50-7, 1992.
Barnett MJ, Sutherland HJ, Eaves AC, Hogge DE, Humphries RK, Klingemann HG, Lansdorp PM, Phillips GL, Reece DE, Shepherd JD, & et al. Human hematopoietic stem cells in long-term culture: quantitation and manipulation. Bone Marrow Transplant 7 Suppl 1: 70, 1991.
Dougherty GJ, Landorp PM, Cooper DL, & Humphries RK. Molecular cloning of CD44R1 and CD44R2, two novel isoforms of the human CD44 lymphocyte “homing” receptor expressed by hemopoietic cells. J Exp Med 174 (1): 1-5, 1991.
Eaves CJ, Cashman JD, Kay RJ, Dougherty GJ, Otsuka T, Gaboury LA, Hogge DE, Lansdorp PM, Eaves AC, & Humphries RK. Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term human marrow cultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer. Blood 78 (1): 110-7, 1991.
Eaves CJ, Cashman JD, Sutherland HJ, Otsuka T, Humphries RK, Hogge DE, Lansdorp PL, & Eaves AC. Molecular analysis of primitive hematopoietic cell proliferation control mechanisms. Ann N Y Acad Sci 628: 298-306, 1991.
Eaves CJ, Sutherland HJ, Cashman JD, Otsuka T, Lansdorp PM, Humphries RK, Eaves AC, & Hogge DE. Regulation of primitive human hematopoietic cells in long-term marrow culture. Semin Hematol 28 (2): 126-31, 1991.
Hogge DE, Cashman JD, Humphries RK, & Eaves CJ. Differential and synergistic effects of human granulocyte-macrophage colony-stimulating factor and human granulocyte colony-stimulating factor on hematopoiesis in human long-term marrow cultures. Blood 77 (3): 493-9, 1991.
Kay R, Rosten PM, & Humphries RK. CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol 147 (4): 1412-6, 1991.
Otsuka T, Eaves CJ, Humphries RK, Hogge DE, & Eaves AC. Lack of evidence for abnormal autocrine or paracrine mechanisms underlying the uncontrolled proliferation of primitive chronic myeloid leukemia progenitor cells. Leukemia 5 (10): 861-8, 1991.
Otsuka T, Humphries RK, Hogge DE, Eaves AC, & Eaves CJ. Continuous activation of primitive hematopoietic cells in long-term human marrow cultures containing irradiated tumor cells. J Cell Physiol 148 (3): 370-9, 1991.
St Amand J, Beard C, Humphries K, & Astell CR. Analysis of splice junctions and in vitro and in vivo translation potential of the small, abundant B19 parvovirus RNAs. Virology 183 (1): 133-42, 1991.
Turhan AG, Eaves CJ, Humphries RK, Phillips GL, & Eaves AC. Reversing clonality in leukemia. Semin Hematol 28 (3 Suppl 4): 5-8, 1991.
Fraser CC, Eaves CJ, Szilvassy SJ, & Humphries RK. Expansion in vitro of retrovirally marked totipotent hematopoietic stem cells. Blood 76 (6): 1071-6, 1990.
Iyer J, Duerst RE, Looney JN, Humphries RK, Abboud CN, & Frantz CN. An 80,000-kd glycoprotein cell surface antigen found only on nonhematopoietic cells in human bone marrow. Exp Hematol 18 (5): 384-9, 1990.
Kay R, Dougherty GJ, & Humphries RK. Heterogeneous modifications of the 114/A10 protein of interleukin-3-dependent cells are concentrated in a highly repetitive amino-terminal domain. J Biol Chem 265 (9): 4962-8, 1990.
Kay R, Takei F, & Humphries RK. Expression cloning of a cDNA encoding M1/69-J11d heat-stable antigens. J Immunol 145 (6): 1952-9, 1990.
Szilvassy SJ, Humphries RK, Lansdorp PM, Eaves AC, & Eaves CJ. Quantitative assay for totipotent reconstituting hematopoietic stem cells by a competitive repopulation strategy. Proc Natl Acad Sci U S A 87 (22): 8736-40, 1990.
Turhan AG, Humphries RK, Eaves CJ, Barnett MJ, Phillips GL, Kalousek DK, Klingemann HG, Lansdorp PL, Reece DE, Shepherd JD, & et al. Detection of breakpoint cluster region- negative and nonclonal hematopoiesis in vitro and in vivo after transplantation of cells selected in cultures of chronic myeloid leukemia marrow. Blood 76 (11): 2404-10, 1990.
Wognum AW, Lansdorp PM, Humphries RK, & Krystal G. Detection and isolation of the erythropoietin receptor using biotinylated erythropoietin. Blood 76 (4): 697-705, 1990.
Dougherty GJ, Dougherty ST, Kay RJ, Landsdorp P, & Humphries RK. Identification and characterization of 114/A10, an antigen highly expressed on the surface of murine myeloid and erythroid progenitor cells and IL-3-dependent cell lines. Exp Hematol 17 (8): 877-82, 1989.
Dougherty GJ, Kay RJ, & Humphries RK. Molecular cloning of 114/A10, a cell surface antigen containing highly conserved repeated elements, which is expressed by murine hemopoietic progenitor cells and interleukin-3-dependent cell lines. J Biol Chem 264 (11): 6509-14, 1989.
Hughes PF, Eaves CJ, Hogge DE, & Humphries RK. High-efficiency gene transfer to human hematopoietic cells maintained in long-term marrow culture. Blood 74 (6): 1915-22, 1989.
Szilvassy SJ, Fraser CC, Eaves CJ, Lansdorp PM, Eaves AC, & Humphries RK. Retrovirus-mediated gene transfer to purified hemopoietic stem cells with long-term lympho-myelopoietic repopulating ability. Proc Natl Acad Sci U S A 86 (22): 8798-802, 1989.
Szilvassy SJ, Lansdorp PM, Humphries RK, Eaves AC, & Eaves CJ. Isolation in a single step of a highly enriched murine hematopoietic stem cell population with competitive long-term repopulating ability. Blood 74 (3): 930-9, 1989.
Turhan AG, Humphries RK, Phillips GL, Eaves AC, & Eaves CJ. Clonal hematopoiesis demonstrated by X-linked DNA polymorphisms after allogeneic bone marrow transplantation. N Engl J Med 320 (25): 1655-61, 1989.
Fraser C, Humphries RK, & Mager DL. Chromosomal distribution of the RTVL-H family of human endogenous retrovirus-like sequences. Genomics 2 (4): 280-7, 1988.
Humphries RK, Abraham S, Krystal G, Lansdorp P, Lemoine F, & Eaves CJ. Activation of multiple hemopoietic growth factor genes in Abelson virus-transformed myeloid cells. Exp Hematol 16 (9): 774-81, 1988.
Lemoine FM, Humphries RK, Abraham SD, Krystal G, & Eaves CJ. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Exp Hematol 16 (8): 718-26, 1988.
Lemoine FM, Krystal G, Humphries RK, & Eaves CJ. Autocrine production of pre-B-cell stimulating activity by a variety of transformed murine pre-B-cell lines. Cancer Res 48 (22): 6438-43, 1988.
Turhan AG, Eaves CJ, Kalousek DK, Eaves AC, & Humphries RK. Molecular analysis of clonality and bcr rearrangements in Philadelphia chromosome-positive acute lymphoblastic leukemia. Blood 71 (5): 1495-8, 1988.
Turhan AG, Humphries RK, Cashman JD, Cuthbert DA, Eaves CJ, & Eaves AC. Transient suppression of clonal hemopoiesis associated with pregnancy in a patient with a myeloproliferative disorder. J Clin Invest 81 (6): 1999-2003, 1988.
Hogge DE, & Humphries RK. Gene transfer to primary normal and malignant human hemopoietic progenitors using recombinant retroviruses. Blood 69 (2): 611-7, 1987.
Wong PM, Humphries RK, Chen TR, & Eaves CJ. Evidence for a multistep pathogenesis in the generation of tumorigenic cell lines from hemopoietic colonies exposed to Abelson virus in vitro. Exp Hematol 15 (3): 280-7, 1987.
Dover GJ, Humphries RK, Moore JG, Ley TJ, Young NS, Charache S, & Nienhuis AW. Hydroxyurea induction of hemoglobin F production in sickle cell disease: relationship between cytotoxicity and F cell production. Blood 67 (3): 735-8, 1986.
Moore JG, Humphries RK, Frank MM, & Young N. Characterization of the hematopoietic defect in paroxysmal nocturnal hemoglobinuria. Exp Hematol 14 (3): 222-9, 1986.
Anderson WF, Goldberg S, Kantoff P, Berg P, Eglitis M, & Humphries RK. Attempts at gene therapy in beta-thalassemic mice. Ann N Y Acad Sci 445: 445-51, 1985.
Dover GJ, Humphries RK, Young N, Ley T, Boyer S, Charache S, & Nienhuis A. Pharmacologic manipulation of fetal hemoglobin synthesis. Prog Clin Biol Res 191: 447-54, 1985.
Greenberger JS, Humphries RK, Messner H, Reid DM, & Sakakeeny MA. Molecularly cloned and expressed murine T-cell gene product is biologically similar to interleukin-3. Exp Hematol 13 (4): 249-60, 1985.
Humphries RK, Berg P, DiPietro J, Bernstein S, Baur A, Nienhuis AW, & Anderson WF. Transfer of human and murine globin-gene sequences into transgenic mice. Am J Hum Genet 37 (2): 295-310, 1985.
Humphries RK, Dover G, Young NS, Moore JG, Charache S, Ley T, & Nienhuis AW. 5-Azacytidine acts directly on both erythroid precursors and progenitors to increase production of fetal hemoglobin. J Clin Invest 75 (2): 547-57, 1985.
Karlsson S, Humphries RK, Gluzman Y, & Nienhuis AW. Transfer of genes into hematopoietic cells using recombinant DNA viruses. Proc Natl Acad Sci U S A 82 (1): 158-62, 1985.
Nienhuis AW, Ley TJ, Humphries RK, Young NS, & Dover G. Pharmacological manipulation of fetal hemoglobin synthesis in patients with severe beta-thalassemia. Ann N Y Acad Sci 445: 198-211, 1985.
Chen MJ, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, & Nienhuis AW. The functional human dihydrofolate reductase gene. J Biol Chem 259 (6): 3933-43, 1984.
Humphries RK, Ley TJ, Anagnou NP, Baur AW, & Nienhuis AW. Beta O-39 thalassemia gene: a premature termination codon causes beta-mRNA deficiency without affecting cytoplasmic beta-mRNA stability. Blood 64 (1): 23-32, 1984.
Humphries RK, & Young N. Aplastic anemia and stem cell biology. Prog Clin Biol Res 148: 3-12, 1984.
Young N, Harrison M, Moore J, Mortimer P, & Humphries RK. Direct demonstration of the human parvovirus in erythroid progenitor cells infected in vitro. J Clin Invest 74 (6): 2024-32, 1984.
Young NS, Moore JG, & Humphries RK. The human parvovirus and in vitro hematopoietic colony formation. Prog Clin Biol Res 148: 129-39, 1984.
Young NS, Mortimer PP, Moore JG, & Humphries RK. Characterization of a virus that causes transient aplastic crisis. J Clin Invest 73 (1): 224-30, 1984.
Zoumbos NC, Ferris WO, Hsu SM, Goodman S, Griffith P, Sharrow SO, Humphries RK, Nienhuis AW, & Young N. Analysis of lymphocyte subsets in patients with aplastic anaemia. Br J Haematol 58 (1): 95-105, 1984.
Cashman J, Henkelman D, Humphries K, Eaves C, & Eaves A. Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny. Blood 61 (5): 876-84, 1983.
Dean A, Ley TJ, Humphries RK, Fordis CM, Jr., & Schechter AN. Abundance and structure of globin mRNA in K562 human leukemia cells. Prog Clin Biol Res 134: 323-34, 1983.
Dean A, Ley TJ, Humphries RK, Fordis M, & Schechter AN. Inducible transcription of five globin genes in K562 human leukemia cells. Proc Natl Acad Sci U S A 80 (18): 5515-9, 1983.
Goldsmith ME, Humphries RK, Ley T, Cline A, Kantor JA, & Nienhuis AW. “Silent” nucleotide substitution in a beta+-thalassemia globin gene activates splice site in coding sequence RNA. Proc Natl Acad Sci U S A 80 (8): 2318-22, 1983.
Greenberger JS, Sakakeeny MA, Humphries RK, Eaves CJ, & Eckner RJ. Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines. Proc Natl Acad Sci U S A 80 (10): 2931-5, 1983.
Humphries RK, Ley T, Goldsmith ME, Kantor JA, Cline AC, & Nienhuis AW. “Silent” nucleotide substitution in codon 24 of a beta+ thalassemia globin gene activates splice site in coding sequence RNA. Prog Clin Biol Res 134: 123-6, 1983.
Mortimer PP, Humphries RK, Moore JG, Purcell RH, & Young NS. A human parvovirus-like virus inhibits haematopoietic colony formation in vitro. Nature 302 (5907): 426-9, 1983.
Humphries RK, Ley T, Turner P, Moulton AD, & Nienhuis AW. Differences in human alpha-, beta- and delta-globin gene expression in monkey kidney cells. Cell 30 (1): 173-83, 1982.
Ley TJ, DeSimone J, Anagnou NP, Keller GH, Humphries RK, Turner PH, Young NS, Keller P, & Nienhuis AW. 5-azacytidine selectively increases gamma-globin synthesis in a patient with beta+ thalassemia. N Engl J Med 307 (24): 1469-75, 1982.
Humphries RK, Eaves AC, & Eaves CJ. Self-renewal of hemopoietic stem cells during mixed colony formation in vitro. Proc Natl Acad Sci U S A 78 (6): 3629-33, 1981.
Eaves CJ, Humphries RK, & Eaves AC. Marrow flask cultures—a system for examining early erythropoietic differentiation events. Blood Cells 5 (3): 377-87, 1979.
Humphries RK, Eaves AC, & Eaves CJ. Characterization of a primitive erythropoietic progenitor found in mouse marrow before and after several weeks in culture. Blood 53 (4): 746-63, 1979.
Humphries RK, Jacky PB, Dill FJ, Eaves AC, & Eaves CJ. CFU-S in individual erythroid colonies derived in vitro from adult mouse marrow. Nature 279 (5715): 718-20, 1979.
Humphries RK, & Miller RG. Volume analysis of human peripheral blood leukocytes. Ser Haematol 5 (2): 142-62, 1972.