Phil Hieter, PhD
| Professor |
Selected Publications:
Ben-Aroya, S., Agmon, N., Yuen, K., Kwok, T., McManus, K., Kupiec, M., Hieter, P. (2010). Proteasome nuclear activity affects chromosome stability by controlling the turnover of Mms22, a protein important for DNA repair. PLoS Genetics 6(2):e1000852.
Fridman,Y., Palgi, N., Dovrat, D., Ben-Aroya, S., Hieter, P., Aharoni, A. (2010) Subtle alterations in PCNA-partner interactions severely impair DNA replication and repair. PLoS Biology 8(10): e1000507.
McLellan, J., O'Neil. N., Tarailo, S., Stoepel, J., Bryan, J., Rose, A., Hieter, P. (2009). Synthetic lethal genetic interactions that decrease somatic cell proliferation in C. elegans identify the alternative RFC-CTF18 as a candidate cancer drug target. Mol. Biol. Cell 20(24):5306-13.
Ben-Aroya, S., Pan, X., Boeke, J., Hieter, P. (2009). Making temperature sensitive mutants. Methods in Enzymology 470:181-204.
McManus, K., Barrett, I., Nouhi, Y., Hieter, P. (2009) Specific synthetic lethal killing of RAD54B-deficient human colorectal cancer cells by FEN1 silencing. Proc. Natl. Acad. Sci. USA. 106(9):3276-81.
McManus, K., Barrett, I., Nouhi, Y., Hieter, P. (2009) Specific synthetic lethal killing of RAD54B-deficient human colorectal cancer cells by FEN1 silencing. Proc. Natl. Acad. Sci. USA. In press.
Rogic, S., Montpetit, B., Hoos, H.H., Mackworth, A.K., Ouellette, F.B., Hieter, P. (2008) Correlation between the secondary structure of pre-mRNA introns and the efficiency of splicing in Saccharomyces cerevisiae.
Rogic, S., Montpetit, B., Hoos, H.H., Mackworth, A.K., Ouellette, F.B., Hieter, P. (2008) Correlation between the secondary structure of pre-mRNA introns and the efficiency of splicing in Saccharomyces cerevisiae. BMC Genomics 9:355.
Ben-Aroya, S., Coombes, C., Kwok, T., O’Donnell, K., Boeke, J., Hieter, P. (2008) Towards a comprehensive temperature sensitive mutant repository for all essential genes of the yeast Saccharomyces cerevisiae. Molecular Cell. 30(2):248-58.
Barber T.D., McManus K., Yuen K.W., Reis M., Parmigiani G., Shen D., Barrett I., Nouhi Y., Spencer F., Markowitz S., Velculescu V.E., Kinzler K.W., Vogelstein B., Lengauer C., Hieter P. (2008) Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers. Proc. Natl. Acad. Sci. USA. 105(9):3443-8.
Yuen, K., Warren, C., Chen, O., Kwok, T., Hieter, P., Spencer, F. (2007) Systematic genome instability screens in yeast and their potential relevance to cancer. Proc. Natl. Acad. Sci. USA. 104(10):3925-30.
Collins, R., Miller, K., Maas, N., Roguev, A., Schuldiner, M., Gebbia, M., Cheng, B., Recht, J., Fillingham, J., Ingvarsdottir, K., Andrews, B., Boone, C., Emili, A., Allis, C.D., Berger, S.L., Brown, G.W., Hieter, P., Zhang, Z., Toczyski, D.P., Greenblatt, J.F., Weissman, J.S. Krogan, J.N. (2007) Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. Nature 446(7137):806-10.
Martin, D.G., Baetz, K., Shi, X., Walter, K., Wlodarski, M., Gozani, O., Hieter, P., Howe, L. (2006) The Yng1p PHD Finger is a Methyl-Histone Binding Module that Recognizes Lysine 4 Methylated Histone H3. Mol Cell Biol. 26:7871-7879
Spradling, A., Ganetsky, B., Hieter, P., Johnston, M., Olson, M., Orr-Weaver, T., Rossant, J., Sanchez, A., Waterston, R. (2006) New roles for model genetic organisms in understanding and treating human disease. Genetics 172:2025-32
Montpetit, B., Hazbun, T., Fields, S., Hieter, P. (2006) Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and anaphase spindle elongation. J. Cell Biol. 174(5):653-63
Yuen K.W., Montpetit B., Hieter P. (2005) Abstract The kinetochore and cancer: what's the connection? Curr Opin Cell Biol. Dec;17(6):576-82
Pot I., Knockleby J., Aneliunas V., Nguyen T., Ah-Kye S., Liszt G., Snyder M., Hieter P., Vogel J. (2005) Spindle checkpoint maintenance requires Ame1 and Okp1.Cell Cycle. Oct;4(10):1448-56
Stoepel J., Ottey M.A., Kurischko C., Hieter P., Luca F.C. (2005) The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Mol Biol Cell. Dec;16(12):5465-79
Measday, V., Baetz, K., Guzzo, J., Yuen, K., Kwok, T., Sheikh, B., Ding, H., Ueta, R., Hoac, T., Cheng, B., Pot, I., Tong, A., Yamaguchi-Iwai, Y., Boone, C., Hieter, P., Andrews, B. (2005) Genomic Dissection of the Yeast Kinetochore Identifies a Role for the Iron Responsive Transcription Factor Rcs1 in Chromosome Stability. Proc. Natl. Acad. Sci. USA. Sep 27;102(39):13956-61
Montpetit, B., Thorne, K., Barrett, I., Andrews, K., Jadusingh, R., Sheikh, B., Hieter, P., Measday, V. (2005) Genome wide synthetic lethal screening with kinetochore mutants identifies a role for the mRNA export protein Apq12 in cell cycle progression. Genetics. Oct;171(2):489-501
Page, A., Aneliunas, V., Lamb, J., Hieter, P. (2005) In vivo characterization of non-essential budding yeast APC/C components Swm1p, Mnd2p, and Apc9p. Genetics. 170(3):1045-62
Yi E.C., Li X.J., Cooke K., Lee H., Raught B., Page A., Aneliunas V., Hieter P., Goodlett D.R., Aebersold R. (2005) Increased quantitative proteome coverage with (13)C/(12)C-based, acid-cleavable isotope-coded affinity tag reagent and modified data acquisition scheme. Proteomics 5(2):380-7
Pan, X., Yuan, D., Xiang, D., Wang, X., Sookhai-Mahadeo, S., Bader, J., Hieter, P., Spencer, F., and Boeke, J. (2004) A robust toolkit for functional profiling of the yeast genome. Molecular Cell. 16(3):487-96
Krogan, N.J., Baetz, K., Keogh, M.C., Datta, N., Sawa, C., Kwok, T.C., Thompson, N.J., Davey, M.G., Pootoolal, J., Hughes, T.R., Emili, A., Buratowski, S., Hieter, P., and Greenblatt, J.F. (2004) Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4. Proc Natl. Acad. Sci. 101(37):13513-8
Baetz, K., McHardy, L., Gable, K., Tarling, T., Rebérioux, T., Bryan, J., Andersen, R., Dunn, T., Hieter, P., Roberge, R. (2004) Yeast genome-wide drug-induced haploinsufficiency screen to determine drug mode of action. Proc Natl Acad Sci. 101(13):4525-4530
Measday, V., Hieter, P. (2004) Kinetochore sub-structure comes to MIND. Nat Cell Biol. 6(2):94-95
Mayer, M., Pot, I., Chang, M., Xu, H., Aneliunas, V., Kwok, T., Newitt, R., Aebersold, R., Boone, C., Brown, G., and Hieter, P. (2004) Identification of Protein Complexes Required for Efficient Sister Chromatid Cohesion. Mol Biol Cell 15(4):1736-1745
Baetz, K., Krogan, N., Emili, A., Greenblatt, J., and Hieter, P. (2004) ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC which is required for the establishment of sister chromatid cohesion. Mol. Cell. Biol. 24: 1232-1244
Kitagawa K., Abdulle R., Bansal P.K., Cagney G., Fields S., Hieter P. (2003) Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Mol. Cell. 11(5):1201-13
Pot, I., Measday, V., Snydsman, B., Cagney, G., Fields, S., Davis, T.N., Muller, E.G., and Hieter, P. (2003) Chl4p and iml3p are two new members of the budding yeast outer kinetochore. Mol. Biol. Cell. 14:460-476
Measday, V., Hailey, D. W., Pot, I., Givan, S. A., Hyland, K.M., Cagney, G., Fields, S., Davis, T. N., and Hieter, P. (2002) Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev. 16: 101-13
Kitagawa, K. and Hieter, P. (2001) Evolutionary conservation between budding yeast and human kinetochores. Nat. Rev. Mol. Cell. Biol. 2: 678-87
Mayer, M. L., Gygi, S. P., Aebersold, R., and Hieter, P. (2001) Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae. Mol. Cell. 7: 959-70
Kinoshita, Y., Jarell, A. D., Flaman, J. M., Foltz, G., Schuster, J., Sopher, B. L., Irvin, D. K., Kanning, K., Kornblum, H. I., Nelson, P. S., Hieter, P., and Morrison, R. S. (2001) Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells. J. Biol. Chem. 276: 6656-65
Mayer, M. L., and Hieter, P. (2000) Protein networks - built by association. Nat. Biotechnol. 18: 1242-43
Leverson, J. D., Joazeiro, C. A., Page, A. M., Huang, H., Hieter, P., and Hunter, T. (2000) The APC11 RING-H2 finger mediates E2-dependent ubiquitination. Mol. Biol. Cell 11: 2315-25
Page, A. M., and Hieter, P. (1999) The anaphase-promoting complex: new subunits and regulators. Annu. Rev. Biochem. 68: 583-609
Kitagawa, K., Skowyra, D., Elledge, S. J., Harper, J. W., and Hieter, P. (1999) SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. Mol. Cell 4: 21-33
Hyland, K. M., Kingsbury, J., Koshland, D., and Hieter, P. (1999)
Ctf19p: A novel kinetochore protein in Saccharomyces cerevisiae and a potential link between the kinetochore and mitotic spindle. J. Cell Biol. 145: 15-28
Skibbens, R. V., Corson, L. B., Koshland, D., and Hieter, P. (1999) Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machiner". Genes Dev. 13: 307-19
Skibbens, R. V., and Hieter, P. (1998) Kinetochores and the checkpoint mechanism that monitors for defects in the chromosome segregation machinery. Annu. Rev. Genet. 32: 307-37
Page, A. M., and Hieter, P. (1997). The anaphase promoting complex. Cancer Surv. 29: 133-50.